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  • DNA Sorcerer
    Member
    • Mar 2010
    • 24
    #1

    Experience with RNAlater samples?

    I have two tubes of a precious, irreplaceable sample with approximately 20 cells of an unknown eukaryotic microbe and would like to get a transcriptome out of it. As far as I could find out, the way to go is spinning down the cells and proceed with a typical RNA prep.

    I have two important questions before proceeding: Is there any other recommendation for a minute sample stored in RNAlater?

    What is safer: do RNA amplification and then cDNA or do cDNA and then DNA amplification?

    Thanks,
    Hi there
    Tags: None
  • DNA Sorcerer
    Member
    • Mar 2010
    • 24
    #2
    I've decided to use Cells-to-cDNA by Ambion. If someone objects to that please let me know asap!
    Hi there

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