Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Gina_P
    Member
    • Dec 2011
    • 20

    #1

    Anyone know what this peak is on my BA trace?

    This is an RNA pico chip and the well is supposed to be empty.
    Attached Files
    - Gina
  • kwaraska
    Senior Member
    • Nov 2008
    • 131

    #2
    If wells before it are overloaded, it will show up in random wells. Kind of like the capillary sequencers-if one cap is high flourescence and the neighboring shows nothing, the other sequence may appear to be in that cap

    Comment

    • Gina_P
      Member
      • Dec 2011
      • 20

      #3
      Hm. That could be it. It says the previous wells are around 200 pg/uL, so I'm not sure, unless some of that sample didn't even get measured and just went into the empty wells. Would it be bunched together in a peak that is >4000 nt like that?
      - Gina

      Comment

      • kwaraska
        Senior Member
        • Nov 2008
        • 131

        #4
        It can be. When it goes through the capillaries of the chip, it can let go at any time to come out at any size.

        Comment

        • Gina_P
          Member
          • Dec 2011
          • 20

          #5
          Ah I see. Thanks!
          - Gina

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM
          • SEQadmin2
            New Genomics Technologies Take Aim at Long-Standing Limits
            by SEQadmin2


            Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

            We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
            ...
            09-28-2026, 10:25 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 10-22-2026, 11:13 AM
          0 responses
          30 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 10:48 AM
          0 responses
          22 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-15-2026, 10:52 AM
          0 responses
          39 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-14-2026, 05:39 AM
          0 responses
          45 views
          0 reactions
          Last Post SEQadmin2  
          Working...