Sequencing smaller regions in the genome ?

[AmitChaurasia]

Hi Friends,

I am new to sequencing field. I have to sequence 10mb region from the human genome. I do not want to sequence whole genome. Kindly suggest me the ways to proceed.

Best,
Amit

[kmcarr]

You can use the Illumina Custom Enrichment Kit. Using the Illumina DesignStudio a set of capture probes are created to cover your region of interest. Illumina then synthesizes the probes, packages them up with their enrichment kit and ships it to you. Contact the Illumina sales rep or Field Application Scientist in your area.

[krobison]

There are solutions for this from other vendors as well, such as Agilent and Nimblegen and others.

[AmitChaurasia]

thanks dear Kmcarr and krobison for the reply,

I am satisfied with the answers, but now more curious about how efficient the coverage would be and will it cost effective ?

thanks ?