Hey gang, I've got a bunch of samples already sequenced with libraries built with Illumina's truseq v2 kits. I have a few left to do but am running into the small-potatoes user problem of not needing 48 reactions and am thus loathe to buy another full v2 kit. I also have a strong suspicion that NEB provides the enzymes for those kits. So: has anybody here ever tried to compare libraries made by truseq with libraries of the same RNA made with NEBnext? If there's very little bias, I will be sorely tempted to go that route.
Thanks for any insights!
Thanks for any insights!
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