Left my beads on bench overnight. Is it still OK to use?
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The performance shouldn't be affected from sitting at room temperature overnight. The beads are just magnets in polyethylene glycol; there's nothing in terms of enzymes or anything that would degrade. As long as you don't boil them or freeze them they'll last a while.
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i frequently freeze beads under water sup during elution and then thaw, incubate, and remove beads later
if you freeze the raw ampure solution i'm not sure what happens, it's pretty concentrated stuff so there might be some precipitation. or maybe not. if not i bet they're fine.
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Originally posted by elhb View PostHi, what do you think might happen if you by chance happen to freeze them??
Leaving AMPure XP beads on your bench overnight will not affect the performance at all. I have known people who stored beads at room temp for weeks and used them regularly with no problems, although I wouldn't recommend that.
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hello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.
thank you very much
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Originally posted by hssalgh2 View Posthello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.
thank you very much
Comment
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Originally posted by hssalgh2 View Posthello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.
thank you very much
Comment
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Originally posted by Simone78 View PostThere are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it
Regards
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Originally posted by Simone78 View PostThere are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it
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