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Left my beads on bench overnight. Is it still OK to use?
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The performance shouldn't be affected from sitting at room temperature overnight. The beads are just magnets in polyethylene glycol; there's nothing in terms of enzymes or anything that would degrade. As long as you don't boil them or freeze them they'll last a while. -
only potential problem is stuff growing in them and that stuff's DNA getting into whatever library you were making. not a problem for all library prep protocols.Comment
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Thank you.Comment
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Hi, what do you think might happen if you by chance happen to freeze them??Comment
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i frequently freeze beads under water sup during elution and then thaw, incubate, and remove beads later
if you freeze the raw ampure solution i'm not sure what happens, it's pretty concentrated stuff so there might be some precipitation. or maybe not. if not i bet they're fine.Comment
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I have always heard that freezing magnetic beads in general makes them behave "weird," but I don't recall any actual details or evidence.Originally posted by elhb View Post
Leaving AMPure XP beads on your bench overnight will not affect the performance at all. I have known people who stored beads at room temp for weeks and used them regularly with no problems, although I wouldn't recommend that.Comment
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I have personally left Ampure beads out, ON, on accident. Paranoid, I thought I couldn't use them, but I did, and everything was just fine!Comment
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hello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.
thank you very muchComment
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There are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do itOriginally posted by hssalgh2 View Post
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Thank you so much but is it ok when I left beads on the shelf for two weeks not in the friadg that happen by chance!Originally posted by Simone78 View PostThere are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it
RegardsComment
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A qucik look at the SDS (https://www.beckmancoulter.com/wsrpo...entId=45481385 ) confirms that they do.Originally posted by Simone78 View PostThere are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do itComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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