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Hi all,
I made 48 libraries, most of which meet our expectations, (see 4C) with peak FU at ~ 290bp (library is 170bp insert +121 bp adaptor) with a few that have a mysterious second peak (2A, 4A) in the range of 500-700bp. Is this DNA bubble or something else?
As mentioned in a previous thread, "Truseq RNA library has 2 peak on bioanalyzer", which according to Illumina does not adversely affect sequencing. Can the same be said for my samples?
If so, is the DNA bubble an innocuous contaminant to downstream sequencing? If not, what can be done to eliminate the second higher molecular weight peak before I proceed to run my samples on Hi-seq?
Thanks all,
-miniseq
I made 48 libraries, most of which meet our expectations, (see 4C) with peak FU at ~ 290bp (library is 170bp insert +121 bp adaptor) with a few that have a mysterious second peak (2A, 4A) in the range of 500-700bp. Is this DNA bubble or something else?
As mentioned in a previous thread, "Truseq RNA library has 2 peak on bioanalyzer", which according to Illumina does not adversely affect sequencing. Can the same be said for my samples?
If so, is the DNA bubble an innocuous contaminant to downstream sequencing? If not, what can be done to eliminate the second higher molecular weight peak before I proceed to run my samples on Hi-seq?
Thanks all,
-miniseq