Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • seqgirl123
    Member
    • Oct 2008
    • 78

    #1

    Illumina sequences with Kapa kit

    Has anyone used Illumina Truseq adapters and the PPC primer cocktail with the Kapa library preparation kit (Illumina compatible version)? The Kapa kit allows the user to use outside adapter and PCR primer primers, this is the reason why I'm asking, if anyone has tried it and how it worked?

    Also I am asking because I want to try Kapa's HiFi polymerase. But since I plan to use Truseq adapters, I am at a loss to how to synthesize Truseq PCR primers to use along with Kapa's polymerase, and since Illlumina doesn't publish their PCR primer sequences anymore, I don't know what other PCR primers to use (besides qPCR primers) other than the PPC primer cocktail. Has anyone explored this?
  • luc
    Senior Member
    • Dec 2010
    • 469

    #2
    I did try the Kapa kit about a year ago. It worked very well. The one drawback is that it requires the old-style cleanup step between A-tailing and adapter ligation; with the Illumina kit or home-brewed protocols you can carry out the A-tailing in ligation buffer and thus skip one of the cleanup steps. The one kit, that I used was also a bit low on reagents - might have been an accident.
    For the Kapa Hifi mastermix we are using an annealing temp of 63 degrees instead of 65; otherwise everything is standard.

    Info on truseq adapters and primers is available here:
    Last edited by luc; 03-29-2013, 09:44 AM.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    38 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    44 views
    0 reactions
    Last Post SEQadmin2  
    Working...