Hi.
I'm trying to analyse 5mC level during development with Illumina sequencing, but the problem is that 5mC level in each development stage is different from each other.
In some sample (4-cell, 8-cell embryos), almost no 5mC was detected.
For library production, would you start with the same amount of each sample or keep the differences and use the unique amount each sample have?
I'm trying to analyse 5mC level during development with Illumina sequencing, but the problem is that 5mC level in each development stage is different from each other.
In some sample (4-cell, 8-cell embryos), almost no 5mC was detected.
For library production, would you start with the same amount of each sample or keep the differences and use the unique amount each sample have?