Hi
I am doing Ribominus RNA and for precipitation i used sodium acetate and Glycogen, after precipitation i supposed to wash the pellet with 7-% Ethanol but somehow forgot to do, I re suspended the pellet immediately and went through the library prep.
Would that affect the quality of the library? is there anyway i can save the RNA?
I am doing Ribominus RNA and for precipitation i used sodium acetate and Glycogen, after precipitation i supposed to wash the pellet with 7-% Ethanol but somehow forgot to do, I re suspended the pellet immediately and went through the library prep.
Would that affect the quality of the library? is there anyway i can save the RNA?