When viewing RNA-seq data against the genome, there are often short regions that have no reads in them. This is probably caused by fragmentation not being uniform. It causes unavoidable problems for transcript isoform assembly. Has anyone had the opportunity to examine if technical replicates of the same biological sample are made, except for varying the sonication time, and the results of sequencing are pooled, whether that improves RNA-seq assembly ? I hope that fragments that were obliterated would be present in the samples with shorter sonication times, and using a spectrum of sonication times could capture a better representation of fragments.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM