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When viewing RNA-seq data against the genome, there are often short regions that have no reads in them. This is probably caused by fragmentation not being uniform. It causes unavoidable problems for transcript isoform assembly. Has anyone had the opportunity to examine if technical replicates of the same biological sample are made, except for varying the sonication time, and the results of sequencing are pooled, whether that improves RNA-seq assembly ? I hope that fragments that were obliterated would be present in the samples with shorter sonication times, and using a spectrum of sonication times could capture a better representation of fragments.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM