I would like to make a pooled DNA solution and get it sequenced by hiseq 2000 Using just one lane.
The pooled DNA solution contains 12 index adaptors from Truseq LS set B kit.
When I see the protocol, Illumina recommends a certain combination of adaptors such as AD05 with AD12 (if pool 2 samples together). It looks like not all the index adaptors can be pooled together.
I want to know if anyone has tried to sequence a DNA pool which contains all 12 index adaptors ? Can it be done? Or will cause serious problems?
The pooled DNA solution contains 12 index adaptors from Truseq LS set B kit.
When I see the protocol, Illumina recommends a certain combination of adaptors such as AD05 with AD12 (if pool 2 samples together). It looks like not all the index adaptors can be pooled together.
I want to know if anyone has tried to sequence a DNA pool which contains all 12 index adaptors ? Can it be done? Or will cause serious problems?
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