truseq DNA LS library preparation --- index adaptor issue

[weihuameng]

I would like to make a pooled DNA solution and get it sequenced by hiseq 2000 Using just one lane.
The pooled DNA solution contains 12 index adaptors from Truseq LS set B kit.
When I see the protocol, Illumina recommends a certain combination of adaptors such as AD05 with AD12 (if pool 2 samples together). It looks like not all the index adaptors can be pooled together.
I want to know if anyone has tried to sequence a DNA pool which contains all 12 index adaptors ? Can it be done? Or will cause serious problems?

[kmcarr]

Using specific adapter combinations is only an issue when you are doing "low-plex" pooling, meaning you are only pooling 2, 3 or 4 libraries in a lane. Beyond that you can add any other adapter(s) to the mix. You can pool all 12 from the TruSeq set B without a problem.

[mukeshwar]

Yes! i have tried all 12 index (TruSeq LT kit) and found no issue. All adaptor-index are compatible and samples de-multiplex easily. Platform used for sequencing was NexSeq 500.