Ovation RNA-Seq System
Hi Staoudi,
In answer to your questions:
1. Yes, our system is compatible with purified mRNA. We have not characterized the input amounts for mRNA, as our system was validated for total RNA in the range of 500 pg to 100 ng. In theory it should be less, but I'd start in the 5-10 ng range if you have this much material.
2. We do not recommend tRNA as a carrier as this can be efficiently amplified in the Ribo-SPIA process used with the Ovation RNA-Seq System. In addition, glycogen is known to inhibit reverse transcription, so we do not recommend this carrier. We have not tested it extensively with our system, but a possibility is the inert carrier linear polyacrylamide (LPA). The addition of LPA to a nucleic acid precipitation can enhance recovery of small amounts of DNA or RNA.
Best,
Steve
Hi Staoudi,
In answer to your questions:
1. Yes, our system is compatible with purified mRNA. We have not characterized the input amounts for mRNA, as our system was validated for total RNA in the range of 500 pg to 100 ng. In theory it should be less, but I'd start in the 5-10 ng range if you have this much material.
2. We do not recommend tRNA as a carrier as this can be efficiently amplified in the Ribo-SPIA process used with the Ovation RNA-Seq System. In addition, glycogen is known to inhibit reverse transcription, so we do not recommend this carrier. We have not tested it extensively with our system, but a possibility is the inert carrier linear polyacrylamide (LPA). The addition of LPA to a nucleic acid precipitation can enhance recovery of small amounts of DNA or RNA.
Best,
Steve
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