homogenization of heart tissue for ChIPSeq

frensch

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Join Date: May 2013

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homogenization of heart tissue for ChIPSeq

07-19-2013, 05:24 AM

Hi,
I am trying doing ChIPSeq for mouse heart homogenates (100mg). I changed the protocol conditions for different times, but I always have the same problem, that I see two peaks in the Agilent Bioanalyzer, the first required peak with ca.200bp and a big peak at >2kb.
I looked for different Cycle conditions, but I guess that I get mistakes in my sample preparation. How can I homogenize and handle the heart tissue in the right way?
Thanks!
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