I am relatively new to RNA seq. I have done some mouse tissue RNA seq and thought mouse RNA seq would be much more difficult than bacterial RNA seq. But I am WRONG!! I have used zymo direct-zol RNA kit to extract RNA from bacteria and it gave very good reading on nanodrop. I know nanodrop reading does not really indicate any quality. I got this kind of reading on bioanalyzer and the RIN number is only 3 and 23S/16S is around 2. does anyone know what's the problem here? thank you very much!
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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