Ampure upper size limit?

[robertami]

"The size of fragments eluted from the beads (or that bind in the first place) is determined by the concentration of PEG, and this in turn is determined by the mix of DNA and beads. A 50ul DNA sample plus 50ul of beads will give a SPRINA ratio of 1, as will 5ul pipetting (but much harder to get right). As this ratio is changed the length of fragments binding and/or left in solution also changes, the lower the ratio of SPRINA the higher larger the final fragments will be at elution"

Maybe this link can be of help

http://core-genomics.blogspot.co.uk/2012/04/how-do-spri-beads-work.html

BW

Roberta

[clintp]

Does anyone know if there is an upper size limitation to the DNA that will precipitate and be bound by Ampure XP beads? I'm specifically thinking of cleaning up Phi29 WGA reactions and long cDNAs.