Fatty Liver Tissue ChIP Seq

[leekb]

Hi all,

I'm doing tissue ChIP seq using human liver tissue both in tumor and adjacent non tumor. I found that the liver tumor tissue always with so much fat/oil droplet and it seriously affect my ChIP so can't get enough DNA after pull down.

Anyone can give me suggestions on how to deal with this problem? The tissues' weight always below 0.2g, I think trim the fat before ChIP not feasible as the fat stay inside the cells.

And we interest in some TFs may not very abundant though.

Thanks in advance!

[doublestrand7]

We have done lots of ChIP-Seq from liver and even from tumor and adjacent normal. These samples have never been a problem and never had problems with fat. If you can not get it to work Active Motif has an end-to-end ChIP-Seq service. http://www.activemotif.com/catalog/8...encing-service

[leekb]

We have done lots of ChIP-Seq from liver and even from tumor and adjacent normal. These samples have never been a problem and never had problems with fat. If you can not get it to work Active Motif has an end-to-end ChIP-Seq service. http://www.activemotif.com/catalog/8...encing-service

Hi doublestrand7,
Nice to hear reply! Would you mind share a little bit more information about how many tissue you are using for IP? I'm doing AR ChIP-seq but sounds like not too much DNA pull down only 7ng using 50mg tissue both adj and tumor.

Many thanks!