I am in process of preparing libraries with 10 ng and 100 ng mRNA for RNA-Seq. I ran a BioA chip (DNA HS) following second strand synthesis and bead purification. However my BioA traces are flat? At this level of mRNA input would I expect to see a library peak following ds cDNA? I just wanted a quick QC before proceeding along the LC process. Any ideas are welcome!
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Maybe looking at this thread might give you some insight?
http://seqanswers.com/forums/showthread.php?t=2003
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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