Has anyone run BioAnalyzer chips on total RNA isolated from nuclei? I am seeing a broad peak at around 5000 bases and nothing that looks like an 18S peak and am not sure what to make of it. (This is from drosophila nuclei, so for cytoplasmic rRNA, most or all of the 28S is cleaved into pieces the same size as the 18S...)
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Phillip
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Phillip
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