We are planning to start a targeted-NGS study of a panel of genes on a GAIIx instrument.
We would be very pleased to have your suggestions on the possible strategies which could be more suitable for our needs. The estimated length of our target regions is about 1.5 Mb. Since we plan to study a large number of patients and we don't have a Covaris for genomic DNA fragmentation, we would like to avoid mechanical fragmentation methods.
Therefore, we are evaluating enzymatic or tagmentation-based systems, such as Agilent HaloPlex Custom kit or the Nextera Rapid Capture Custom Enrichment kit. Which one would you recommend?
Do you know additional targeted enrichment kit, from other companies, suitable for our needs?
Thank you very much for the help!
We would be very pleased to have your suggestions on the possible strategies which could be more suitable for our needs. The estimated length of our target regions is about 1.5 Mb. Since we plan to study a large number of patients and we don't have a Covaris for genomic DNA fragmentation, we would like to avoid mechanical fragmentation methods.
Therefore, we are evaluating enzymatic or tagmentation-based systems, such as Agilent HaloPlex Custom kit or the Nextera Rapid Capture Custom Enrichment kit. Which one would you recommend?
Do you know additional targeted enrichment kit, from other companies, suitable for our needs?
Thank you very much for the help!