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Does anybody 'sizeselect' after the shearing step? How efficient is this compared to not sizeselecting your fragment?
We use the Covaris, and depending on the sample we get from a customer, the 200bp protocol may shear the DNA spot on (from 200-250bp), or give a shearing smear of 100-400bp.In the last case, I was thinking of sizeselecting out the band from 200-250bp. I know one advantage of sizeselecting out after the shearing step is it may reduce chimeric fragments from forming through the library prep process. But are there any pitfalls to this in the downstream processing?
We use the Covaris, and depending on the sample we get from a customer, the 200bp protocol may shear the DNA spot on (from 200-250bp), or give a shearing smear of 100-400bp.In the last case, I was thinking of sizeselecting out the band from 200-250bp. I know one advantage of sizeselecting out after the shearing step is it may reduce chimeric fragments from forming through the library prep process. But are there any pitfalls to this in the downstream processing?