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Hello everyone! Does someone have experience in sequencing amplicon libraries obtained from FFPE? I've heard that there are some specific Taq polimerase that can be used when amplifying FFPE (for example RESTORASE). I was wondering if these Taq are reliable and high-fidelity and if they can be used to prepare amplicon libraries to be sequenced on a 454 GS Junior platform. Does Anyone want to share some experience? Thanks
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...