We are currently performing lib prep using nimblegen's SeqCap EZ whole exome capturing kit.
At the hybridisation step in the protocol, we have to incubate the samples at 47°C for 64-72 hours.
For practical reasons, we can only continue the protocol after 4 days instead of the proposed 3.
We have several options:
1) hybridise the sample for 96 hours
2) hybridise samples for 72 hours and keep them at 4°C untill we are ready to proceed
3) postpone the hybridisation step
As we want to sequence the samples ASAP, we prefer option 1 or 2.
Does anyone have experience with this? Please share.
At the hybridisation step in the protocol, we have to incubate the samples at 47°C for 64-72 hours.
For practical reasons, we can only continue the protocol after 4 days instead of the proposed 3.
We have several options:
1) hybridise the sample for 96 hours
2) hybridise samples for 72 hours and keep them at 4°C untill we are ready to proceed
3) postpone the hybridisation step
As we want to sequence the samples ASAP, we prefer option 1 or 2.
Does anyone have experience with this? Please share.
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