Hello all,
I have recently used the Nextera Mate Pair Sample Preparation Kit
(FC-132-1001) with genomic DNA samples from Helicobacter pylori and
Caulobacter crescentus.
The Caulobacter crescentus showed results as expected whereas
the Helicobacter Pylori showed no results.
Troubleshooting: I ran all samples on the Fragment analyzer from advanced analytical following size selection and they all have material at the right size, but no product after PCR.
Any advice on that? Does anyone notice a fragmentation problem with Helicobacter Pylori?
Thanks,
BMC
I have recently used the Nextera Mate Pair Sample Preparation Kit
(FC-132-1001) with genomic DNA samples from Helicobacter pylori and
Caulobacter crescentus.
The Caulobacter crescentus showed results as expected whereas
the Helicobacter Pylori showed no results.
Troubleshooting: I ran all samples on the Fragment analyzer from advanced analytical following size selection and they all have material at the right size, but no product after PCR.
Any advice on that? Does anyone notice a fragmentation problem with Helicobacter Pylori?
Thanks,
BMC