DNA ng to pM help

[mdalessio]

Sorry to bother everyone, but I am having problems with my conversion.

So, my sample has 0.05 ng/ul

This is the same as 0.05 kg/L

0.05 kg/L x 1 mol / 330 kg [based on 1 kb length ssDNA]

= 0.000151 mol/L

I have 100 ul of this so:

0.000151 mol/L x 0.0001 L = 1.51 x 10^-8 M = 15.1 nM

When I take 24 ul of this pooled sample, and add it to 576 ul of buffer, does that mean that I have...

15.1nM x 0.000024 L = 0.0003624 nmoles

0.0003624 nmoles / 0.0006 L (600 ul) = 2.17 x 10 ^ -7 nM

so that is putting me at 0.000217 pM? So I need to concentrate my sample more than the kit says?

I want to cluster at around 8 pM. So do I go: x/0.006 = 0.008nM (desired concentration

[kwaraska]

This is what I use

http://www.scienceprimer.com/dna-ng-nm-calculator

[bbeitzel]

.05 ng/ul is the same as .05 mg/L, not .05 kg/L

.05 ng/ul works out to be 150 pM