Looking for optamized globinclear protocol

[subxero]

I was wondering if any one has a good Globin reduction protocol for human RNA they are willing to share?

We currently use the LifeTech GLOBINclear kit and it works well but calls for 1.5ug of RNA to start with. We would like to reduce this amount and before I start experimenting I figured I would see if anyone has tried to optimize the LifeTech GLOBINclear protocol or has their own.

[subxero]

^please excuse my typo in thread topic

[woshiliangliang]

hi subxero,
we use also the LifeTech GLOBINclear kit and start with 1ug of total RNA.
proceed exactly as what the kit protocol says.
after the globin mrna removal step,
we use the illumina truseq rna kit to further the library construction.
hope this would help.