I'm interested in doing ribosome profiling on a small bacterial genome. The "standard" protocols all call for isolating ribosomes with either sucrose cushions or (more recently with the Epicentre protocol) S-400 size exclusion. There seems to be an entirely different group doing ribosome isolation using affinity tags on ribosomal proteins, and they call their protocol TRAP. Both seem to get to the same place, but the affinity approaches surely seem easier and more amenable to automation. Does anyone know of why there is a reluctance to use the affinity techniques as an approach to ribosome profiling?
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment