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Palgrave

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Join Date: Aug 2011

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RIP-Seq

11-23-2013, 07:05 AM

Hi,

I am doing RNA-IP on a RNA-binding protein and have the following question:

What is the best way to elute the protein with the RNA from the beads to preserve RNA quality?
I was thinking of using Glycine followed by Phenol extraction of the RNA.
The RNA is not crosslinked to the protein.
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