Hi All,
Does anybody care to comment on the relationship between total RNA quality and sequence quality (specifically Illumina RNA-seq). I have a couple of irreplaceable samples from wild animals in South America that were thawed and exposed to some hot temperatures accidentally. I have a bunch of inter-region signal that I am worried about.. see attached.
Anybody start out with worries re: RNA quality, then get great sequences, or vice versa??
thanks
Does anybody care to comment on the relationship between total RNA quality and sequence quality (specifically Illumina RNA-seq). I have a couple of irreplaceable samples from wild animals in South America that were thawed and exposed to some hot temperatures accidentally. I have a bunch of inter-region signal that I am worried about.. see attached.
Anybody start out with worries re: RNA quality, then get great sequences, or vice versa??
thanks
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