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  • DSN normalization

    Hello,
    We plan to perform Expressed Exome Capture Sequencing (EecSeq) (Puritz and Lotterhos 2018) experiment that includes duplex-specific nuclease (DSN) normalization of cDNA libraries. The DSN kit is produced by Evrogen company (cat.# EA001) that is based in Russia, and there are currently no options to purchase the reagent due to the war embargo. After checking all available options I still don't have an idea where I could get it. Maybe there is someone out there who could share the kit now so I can rebuy it when it's possible?
    Or maybe someone has experience how the normalization step can be replaced?

  • #2
    Hello,
    I currently developed a duplex-specific nuclease for our company as an alternative to the current DSN on the market (made in the USA), PureRec Duplex-Specific Nuclease that is more specific for digesting dsDNA and DNA in DNA-RNA hybrids. It can be used for EecSeq and is available for purchase or a free sample. Please check out Zymo Research (E1020S, E1020-200, and E1020-1000) and I hope this will help you so you can continue your research!

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