DSN normalization

Suslicus

Junior Member

Join Date: Apr 2013

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DSN normalization

09-19-2022, 02:24 AM

Hello,
We plan to perform Expressed Exome Capture Sequencing (EecSeq) (Puritz and Lotterhos 2018) experiment that includes duplex-specific nuclease (DSN) normalization of cDNA libraries. The DSN kit is produced by Evrogen company (cat.# EA001) that is based in Russia, and there are currently no options to purchase the reagent due to the war embargo. After checking all available options I still don't have an idea where I could get it. Maybe there is someone out there who could share the kit now so I can rebuy it when it's possible?
Or maybe someone has experience how the normalization step can be replaced?
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