TET-assisted pyridine borane sequencing(TAPS)

[yizhe2011]

Hello all,

I'm currently interesting in a novel epigenetics sequencing tenology which was named as TET-assisted pyridine borane sequencing (TAPS). This technology converts 5' methylated (5mC) and hydroxymethylated (5hmC) cytosine to 5-formylcytosine (5fC) or 5-carboxylcytosine (5caC) by the ten-eleven translocation (TET) dioxygenases. And 5fC or 5caC were reduced to dihydrouracil (DHU) by pyridine borane. So the method enabling a C-to-T transition of 5mC and 5hmC without affecting unmodified cytosines.

The TAPS method was published on Nature BiotechNology in 2019 and different applications were published on Nature Comminucations, Nucleic Acids Research, Sci. Adv. and Genome Biology.
I think this method is revolutionary in epigenetics and methylation detedtion in NGS.

However, there is no any other lab reports the TAPS since the technology was published. Does anyone has noticed this phenomenon or has experience in this technology?

Thanks!

1.Bisulfite-free direct detection of 5-methylcytosineand 5-hydroxymethylcytosine at base resolution, https:/ / doi.org/10.1038/ s41587-019-0041-2.
2.Whole-genome long-read TAPS deciphers DNA methylation patterns at base resolution using PacBio SMRT sequencing technology, https://doi.org/10.1093/nar/gkac612
3.Cell-free DNA TAPS provides multimodal information for early cancer detection. https://www.science.org/doi/10.1126/sciadv.abh0534
4.Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS. https://doi.org/10.1186/s13059-020-01969-6

[pangcpang]

Yes, you are right. In fact, I was told that many other laboratories have tried to replicate this technique, but have not succeeded in achieving the effects (methylation conversion rate) reported by Professor Song in his paper.