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Hi! I am fairly new with ATAC sequencing and had the following libraries and ths may be a fairly beginner's problem. Can you please tell me if there are any problems in this image or my libraries?
The sequencing facility have advised us that the libraries look good and as expected so we pushed through with the sequencing. However, we found very low amount of peaks after analysis. One reason given to us is that the samples may have been overtagmented. Using this report from the bioanalyzer, will we know the problem and how we will know whether to proceed with the sequencing or not. Thank you very much!
The sequencing facility have advised us that the libraries look good and as expected so we pushed through with the sequencing. However, we found very low amount of peaks after analysis. One reason given to us is that the samples may have been overtagmented. Using this report from the bioanalyzer, will we know the problem and how we will know whether to proceed with the sequencing or not. Thank you very much!