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Interesting because that isn't a lot of cycles. I have had this happen before with another kit many years ago and I'm pretty sure the vendor said I should just lower the PCR cycles. But I also believe that when I sequenced those samples that I still had good data.
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Duuluu I have a few questions to help figure this out.
1. What library prep kit did you use? And did you make any changes to it?
2. How many PCR cycles did you use?
I would guess that your first assumption on the unoptimized PCR is the culprit, but I'm not completely sure.
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bimodal distribution peaks observed in library prep
Hello everyone,
I am seeing two peaks (bimodal distribution) in my lib prep BioAnalyzer. The input sample is gDNA extracted from whole blood. Does anyone what this might be coming from, it looks like a shoulder peak. Could it be due to unoptimized PCR cycles?
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