Hello everyone, I am new to the Seqanswers forum and NGS library preparation. I have prepared a library for WGS of 55 M.tb samples using Illumina DNA prep. All the samples were normalized so that the total DNA input is 100ng per sample and the protocol mentioned in the reference guide was followed as such. After library prep, tape station was performed for 16 samples using D1000 kit. The following are the qubit concentrations and tapestation results. It Would be helpful if anyone could help me interpret the data, whether I can proceed to pool all the samples and submit them for sequencing. The lab we will be outsourcing to will perform the sequencing in Novaseq 6000.
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Last edited by Praveenebenezer; 08-07-2024, 09:06 PM. -
I don't see the traces from the TapeStation. Do you have those? -
Hi callahank, thanks for the reply. Traces can be found in the attached PDF tapestation_traces_0508.pdfComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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