Hello everyone, I am new to the Seqanswers forum and NGS library preparation. I have prepared a library for WGS of 55 M.tb samples using Illumina DNA prep. All the samples were normalized so that the total DNA input is 100ng per sample and the protocol mentioned in the reference guide was followed as such. After library prep, tape station was performed for 16 samples using D1000 kit. The following are the qubit concentrations and tapestation results. It Would be helpful if anyone could help me interpret the data, whether I can proceed to pool all the samples and submit them for sequencing. The lab we will be outsourcing to will perform the sequencing in Novaseq 6000.
-
Last edited by Praveenebenezer; 08-07-2024, 09:06 PM. -
I don't see the traces from the TapeStation. Do you have those? -
Hi callahank, thanks for the reply. Traces can be found in the attached PDF tapestation_traces_0508.pdfComment
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment