Hi there everyone,
I'm performing some chip-seq and have just ligated the adaptors to my product. I sent it to another group with a bioanalyser to test and have just got the data back but it doesnt look too good I fear.
I have a large peak at around 70bp which I am guessing is adaptor carryover. However in a few samples there is another peak at around 125 bp. Is this another adaptor peak or is this my library? I would like to know before i continue and atempt to size select out the 70bp peak.
The bioanalsyer data is attached.
Many thanks.
Ben
I'm performing some chip-seq and have just ligated the adaptors to my product. I sent it to another group with a bioanalyser to test and have just got the data back but it doesnt look too good I fear.
I have a large peak at around 70bp which I am guessing is adaptor carryover. However in a few samples there is another peak at around 125 bp. Is this another adaptor peak or is this my library? I would like to know before i continue and atempt to size select out the 70bp peak.
The bioanalsyer data is attached.
Many thanks.
Ben
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