Polymerase for ChIP-seq. Which do you use?

[amazonic9]

Dear all,

I would like to know which polymerase do you use for your ChIP-seq libraries?

We use the phusion polymerase, but I would like to know your opinion about the other polimerases, and if you have any recommendation for a change.

Thanks a lot in advance.

[Chipper]

Dear all,

I would like to know which polymerase do you use for your ChIP-seq libraries?

We use the phusion polymerase, but I would like to know your opinion about the other polimerases, and if you have any recommendation for a change.

Thanks a lot in advance.

Kapa HiFi
High yield, good for high GC regions, good price.

[amazonic9]

Hi Chipper,

thanks for you answer.

Yesteday we did a test with the NebNext Ultra kit, starting with 1 ng of fragmented DNA, and at the point of the PCR, we tested three polymerases: NebNext HiFi (which comes with the kit), the phusion that we use, and the Kapa HiFi.

And this is the result:



So yes, as you said, at least in terms of yield, Kapa performs much much better than Phusion.

[SS00]

Ditto for KAPA.

Kapa HiFi
High yield, good for high GC regions, good price.