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  • tonybert
    Member
    • Aug 2012
    • 38
    #1

    storage of Amplicon Sequencing products for MiSeq

    Greetings, i have a very basic question. I 18 samples that have been PCR amplified with barcoded primrs, cleaned, and have been stored at 4 degrees C for a few days. Will the quality of the barcodes decrease at 4 degrees versus -20? If anyone has any experience with running these types of experiments, and wouldn't mind sharing, i would appreciate it.

    Thanks,
    Tags: None
    • atcghelix
      Member
      • Jul 2013
      • 74
      #2
      We've been wondering about this too recently. We also have a lot of sunlight in our lab and our 4C fridge has a glass door so we've started to keep libraries in boxes or wrapped in foil. Would be interested to see what others have experienced.

        Comment

        • JamieHeather
          @jamimmunology
          • Nov 2012
          • 96
          #3
          DNA should be fine for months at 4 degrees, provided there's no nuclease contamination.

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