We are interested to try depletion of rRNA from human samples by the RNase H method of Morlan (recommended in the survey of methods by Adiconis):
Morlan JD, Qu K, Sinicropi DV. Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue. PLoS One. 2012;7(8):e42882. doi: 10.1371/journal.pone.0042882.
Adiconis X, Borges-Rivera D, Satija R, DeLuca DS, Busby MA, Berlin AM, Sivachenko A, Thompson DA, Wysoker A, Fennell T, Gnirke A, Pochet N, Regev A, Levin JZ. Comparative analysis of RNA sequencing methods for degraded or
low-input samples. Nat Methods. 2013 Jul;10(7):623-9. doi: 10.1038/nmeth.2483.
I'm wondering if there is a source of the pooled oligos against rRNA and mitochondrial RNA that is required for this method? Of course we can have them custom-synthesized, but would be nice to try it out first.
Anyone have experience with this method that they want to share?
Thanks,
- Peter
Morlan JD, Qu K, Sinicropi DV. Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue. PLoS One. 2012;7(8):e42882. doi: 10.1371/journal.pone.0042882.
Adiconis X, Borges-Rivera D, Satija R, DeLuca DS, Busby MA, Berlin AM, Sivachenko A, Thompson DA, Wysoker A, Fennell T, Gnirke A, Pochet N, Regev A, Levin JZ. Comparative analysis of RNA sequencing methods for degraded or
low-input samples. Nat Methods. 2013 Jul;10(7):623-9. doi: 10.1038/nmeth.2483.
I'm wondering if there is a source of the pooled oligos against rRNA and mitochondrial RNA that is required for this method? Of course we can have them custom-synthesized, but would be nice to try it out first.
Anyone have experience with this method that they want to share?
Thanks,
- Peter
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