RNA-seq Library using ds-cDNA input

[xshang]

Hello,

I'm using REPLI-g WTA Single Cell Kit from Qiagen to amplify cDNA from single cell. I can get double strand cDNA after the kit. What kind of commercial RNA-seq library kit is suit for this material input?

Many thanks!

X.

[kwaraska]

If you have dbl-stranded cDNA you just use a genomic kit from here on out..

You will not get directionality but you still get only transcriptome data