concentration mRNA required

marRoma

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concentration mRNA required

04-29-2014, 07:27 AM

Hello everybody!

i'm struggling with the RNA extraction from bacteria recovered from mice after intraperitoneal infection.... unfortunately i'm not able to get a good amount of total RNA ( the best sample is 256 ng/microliter) and i have around 15 microliter of the sampke) and i'm afraid that the concentration will decrease a lot after the rRNA removal treatment with RiboZero rRNA removal kit.
Does anyone know the concentration of mRNA required for the mRNA sequencing on the Illumina platform?

thank you
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04-30-2014, 06:00 AM

The Epicentre ScriptSeq kit uses starting amounts 0f 500pg - 50ng of rRNA depleted RNA. I think you are in the ballpark.
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marRoma

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05-05-2014, 01:02 AM

ok, thank you very much! fingers cross!
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