Mate pair, high GC

[chen]

It shows that high GC samples may have a lower concentration after PCR.
Is there any notes to take when preparing the mate pair library with the HIGH GC samples, except the PCR process(increase the denature time).
Any suggestion would be help. Thanks.

[nextgen]

Can you try betaine, DMSO to lower Tm?

It shows that high GC samples may have a lower concentration after PCR.
Is there any notes to take when preparing the mate pair library with the HIGH GC samples, except the PCR process(increase the denature time).
Any suggestion would be help. Thanks.

[grace033]

change Enzyme

[Michael.James.Clark]

I guess you're using Phusion HF for your library prep PCR normally. There is a high-GC version of Phusion you could try instead.