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  • Exonuclease I

    Hi,

    I was wondering what do you think of using exonuclease I to get rid of adapter contamination from an RNA-seq library?
    I am using ScriptSeq to make my libraries and although I used AMPure (1x ratio) to purify the library I still had a lot of adapter contamination. I did a 2nd purification (0.9x) which helped but when preparing another library from highly fragmented RNA I used Exonuclease to remove excess PCR primers plus MinElute PCR purification to purify the library as recommended by the kit and it has worked wonders with adapter contamination!
    I was thinking of using Exonuclease I plus AMPure with all my samples.
    Any thoughts?

    Thanks!

  • #2
    That should work. Epicentre includes Exonuclease I in their kit but it's only referred to for use in removing excess PCR primers in FFPE applications. I was told it was holdover from the previous version of the kit.

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