I am working with samples that are mixed Prokaryotic and Eukaryotic Cells, looking at prokaryotic gene expression in food. I don't work with RNA often and would like suggestions on the best method for total RNA extraction (I have only used Trizol in the past with mixed results).
To enrich for the prokaryotic mRNA, after extraction I am planning on using Epicentre's Ribo-Zero™ Magnetic Gold Kit for removal of rRNA.
Any other suggestions for enrichment?
After all that, Illumina has suggested using the TruSeq® Stranded mRNA Sample Preparation.
To enrich for the prokaryotic mRNA, after extraction I am planning on using Epicentre's Ribo-Zero™ Magnetic Gold Kit for removal of rRNA.
Any other suggestions for enrichment?
After all that, Illumina has suggested using the TruSeq® Stranded mRNA Sample Preparation.
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