Hi all,
I have prepared small RNA libraries with the NEBNext kit. In some of my samples, I have a pretty high Adapter dimer contamination (see attached Picture). We have already done AMPure size selection and LabChip (Caliper), but it seems almost impossible to eliminate the dimers since they are very close to our wanted product size (120 vs <130bp).
Do you think that I can still get sufficient read depth with this sample?
Many thanks,
Mona
I have prepared small RNA libraries with the NEBNext kit. In some of my samples, I have a pretty high Adapter dimer contamination (see attached Picture). We have already done AMPure size selection and LabChip (Caliper), but it seems almost impossible to eliminate the dimers since they are very close to our wanted product size (120 vs <130bp).
Do you think that I can still get sufficient read depth with this sample?
Many thanks,
Mona
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