Hi all,
I'm just piggybacking on this thread, but I think it is probably the best place to post my question - and something I can't be the only person having an issue with!
I'm prepping my samples for ATAC-Seq (planning to do single end sequencing 75bp reads, just looking at regions of open chromatin), and I know that most of the protocols have custom primers (both Ad1 and index primers). I was wondering if you HAD to use these, or if there were capable primers in the Nextera library prep kit and index kit to prep the samples. Again, I'm not sure if people use the custom primers because it is cheaper or if it is because Illumina is so vague with their primer mixes and cocktails. Any input would be appreciated!
Cheers
I'm just piggybacking on this thread, but I think it is probably the best place to post my question - and something I can't be the only person having an issue with!
I'm prepping my samples for ATAC-Seq (planning to do single end sequencing 75bp reads, just looking at regions of open chromatin), and I know that most of the protocols have custom primers (both Ad1 and index primers). I was wondering if you HAD to use these, or if there were capable primers in the Nextera library prep kit and index kit to prep the samples. Again, I'm not sure if people use the custom primers because it is cheaper or if it is because Illumina is so vague with their primer mixes and cocktails. Any input would be appreciated!
Cheers
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