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Does anyone have experience using the QIAXCEL to QC libraries? If you could tell me any of the pros or cons of using it I would greatly appreciate it. We'll most likely be using Nextera Kits and running on the MiSeq.
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I plan to, and I am also searching for some one experenced in itOriginally posted by Vanschkn View Post
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QiaXcel compared to Bioanalyzer?
Hello,
I am new to QiaXcel, and I wonder anybody have compared the results between QiaXcel and Bioanalyzer?
Thanks in advance!Comment
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QIAxcel can run libraries for QC, size and concentration
I work as an applications scientist at QIAGEN, and I frequently run libraries on the QIAxcel. Nextera, QIAseq, etc - as long as there was a PCR step during library prep, the QIAxcel can analyze it. I've even started with concentrations <0.1ng/ul.
It's a user-developed application (for now), meaning we have clear instructions how to generate good data, but you'll have to ask someone like your regional sales rep or applications scientist. If you don't know your Q contact, call 1-800-DNA-PREP.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.Yesterday, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before... -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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