Hello all,
I am planning to do Illumina RNA-seq from flow-sorted cells. This means starting the prep with as little as 5 - 10 ng of mRNA.
If possible, I'd like to avoid using the Illumina TruSeq kit and use what we have on hand. This means fragmenting with a bioruptor. Does anyone have suggestions for the sonication settings?
The basic protocol I am following is designed for 10 - 20x more input than I will have. I know the fragmentation conditions scale with input, but I don't know how and I want to be careful with the material I have reserved for testing.
Thanks in advance!
CSO
I am planning to do Illumina RNA-seq from flow-sorted cells. This means starting the prep with as little as 5 - 10 ng of mRNA.
If possible, I'd like to avoid using the Illumina TruSeq kit and use what we have on hand. This means fragmenting with a bioruptor. Does anyone have suggestions for the sonication settings?
The basic protocol I am following is designed for 10 - 20x more input than I will have. I know the fragmentation conditions scale with input, but I don't know how and I want to be careful with the material I have reserved for testing.
Thanks in advance!
CSO
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