Hi all,
I just came across a paper describing a new RAD protocol termed ezRAD (https://peerj.com/articles/203/). In short, you simply digest your DNA with restriction enzymes and then use Illumina's standard TruSeq library preparation kit to make your libraries. Has anyone used this method yet?
I just started a tenure-track position at a teaching college and have limited space, time, and funds to create my own RAD libraries. Floragenex would charge at least $79/sample, which seems pricey to me. I have experience creating ddRAD libraries, but am not sure if this would be feasible at my new institution.
I would welcome suggestions regarding optimal ways to conduct RAD-based population genomic studies at primarily teaching institutions.
All the best,
Chris
I just came across a paper describing a new RAD protocol termed ezRAD (https://peerj.com/articles/203/). In short, you simply digest your DNA with restriction enzymes and then use Illumina's standard TruSeq library preparation kit to make your libraries. Has anyone used this method yet?
I just started a tenure-track position at a teaching college and have limited space, time, and funds to create my own RAD libraries. Floragenex would charge at least $79/sample, which seems pricey to me. I have experience creating ddRAD libraries, but am not sure if this would be feasible at my new institution.
I would welcome suggestions regarding optimal ways to conduct RAD-based population genomic studies at primarily teaching institutions.
All the best,
Chris
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