Hello all--
I work on preparing RAD libraries, and have a question regarding annealing adapters.
Suppose my adapters' top and bottom oligos are each at a stock concentration of 100 uM. If I pool each adapter equi-molarly prior to annealing, will I have 100 uM of annealed adapter or will I have 200 uM of annealed adapter?
Of course, I am simplifying my question, because the thing that is confusing me is the molarity-result of pooling equi-molarly. I have searched for an answer, to no avail! I know this is a basic question, but it has really been stumping me!
Thank you,
Cody
I work on preparing RAD libraries, and have a question regarding annealing adapters.
Suppose my adapters' top and bottom oligos are each at a stock concentration of 100 uM. If I pool each adapter equi-molarly prior to annealing, will I have 100 uM of annealed adapter or will I have 200 uM of annealed adapter?
Of course, I am simplifying my question, because the thing that is confusing me is the molarity-result of pooling equi-molarly. I have searched for an answer, to no avail! I know this is a basic question, but it has really been stumping me!
Thank you,
Cody
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