Hi,
We want to do qPCR quantitating lib. So for the different kinds that I am aware of:
SYBR gree qPCR (From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing)
digital PCR (Digital PCR provides sensitive and absolute calibration for high throughput sequencing)
Taqman qPCR (A large genome center's improvements to the Illumina sequencing system)
Taqman MGB qPCR (Titration-free massively parallel pyrosequencing using trace amounts of starting material)
We are considering KAPA qPCR kit. Anyone uses it and can you share your experience - how much input lib is needed (pmol or copy-per-bead ratio)? Or I have to use one good lib that run well previously as guidance? Do you need to have different 'good lib' for different samples (plant, virus, plasmid, etc)?
Any thoughts appreciated!
We want to do qPCR quantitating lib. So for the different kinds that I am aware of:
SYBR gree qPCR (From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing)
digital PCR (Digital PCR provides sensitive and absolute calibration for high throughput sequencing)
Taqman qPCR (A large genome center's improvements to the Illumina sequencing system)
Taqman MGB qPCR (Titration-free massively parallel pyrosequencing using trace amounts of starting material)
We are considering KAPA qPCR kit. Anyone uses it and can you share your experience - how much input lib is needed (pmol or copy-per-bead ratio)? Or I have to use one good lib that run well previously as guidance? Do you need to have different 'good lib' for different samples (plant, virus, plasmid, etc)?
Any thoughts appreciated!
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